From TechCrunch · Connie Loizos · · 3m
PNOĒ's mask brings breath testing out of the lab
A startup is launching a self-serve metabolic testing device that measures oxygen and carbon dioxide to guide fitness and longevity decisions.
In brief
A startup is launching a self-serve metabolic testing device that measures oxygen and carbon dioxide to guide fitness and longevity decisions. PNOĒ launches a self-administered breath-testing mask on October first that measures metabolic data previously available only in labs, aiming to make lab-grade testing…
A mask that reads your breath

PNOĒ's new device covers the nose and mouth, measuring oxygen consumption and carbon dioxide exhalation to provide advice on eating, training and longevity.
Self-serve testing launches October first
PNOĒ 2.0 launches on October one. Unlike the current device, users can administer the test themselves by wearing the mask, pushing a button and breathing for eight minutes.
“just wear the mask, push the button, sit down”
Based in Massachusetts with Greek operations
PNOĒ is based in Malden, Massachusetts and has operations in Athens, Greece. Co-founder and CEO Apostolos Atsalakis leads the company from its Athens location.
Self-serve opens new venues

The current device requires a trained operator, limiting where it can be used. A self-serve version could open doors to fitness centers without dedicated staff and potentially pharmacies.
Ancient science, modern package
Metabolic testing has existed for more than a century, measuring gases in breath to gauge how the body produces energy. It has been the gold standard for measuring VO two max but required bulky, expensive equipment in labs and hospitals.
Twenty-three biomarkers in one test
PNOĒ's test captures twenty-three biomarkers including VO two max, resting metabolic rate and metabolic flexibility. These metrics answer questions blood tests cannot, such as calorie needs and training approaches.
VO two max and longevity
VO two max has become a buzzword among longevity enthusiasts due to research linking higher cardiorespiratory fitness to lower mortality. Atsalakis calls VO two max the strongest predictor of human longevity.
Designed with Milan firm after many iterations
The new device is smaller and more compact than its predecessor with fewer parts, making it more reliable. It was designed with Milan-based Design Group Italia over what Atsalakis described as a lot, a lot, a lot of iterations.
“a lot, a lot, a lot of iterations”
Shareable hardware, personal masks
Electronics detach from the silicone mask and straps, so multiple people can share the costly hardware while each user keeps their own mask. This addresses post-pandemic concerns about who wore the device last.
Wellness device, not medical
The device is not cleared by the U.S. Food and Drug Administration. PNOĒ considers itself a wellness device and does not provide medical recommendations, saying a doctor cannot prescribe medication based on its results.
“we do not provide medical recommendations”
Raised twenty-two million dollars
PNOĒ went through Y Combinator's Winter two thousand nineteen batch. It has raised about twenty-two million dollars, including a recently closed eleven million dollar round from investors Fifty Years and Google Maps co-founder Lars Rasmussen.
Clients include Equinox and the NBA
PNOĒ sells only to businesses, which offer the test to customers. Clients include Equinox at almost all clubs, Four Seasons hotels, Red Bull, the NBA, Mount Sinai Health System and Restore Hyper Wellness.
Series B planned within six to twelve months
PNOĒ employs one hundred ten people and recently turned profitable while growing more than one hundred percent a year. The company plans to raise a Series B within the next six to twelve months.
The takeaway
PNOĒ launches a self-administered breath-testing mask on October first that measures metabolic data previously available only in labs, aiming to make lab-grade testing accessible to gyms, spas and other businesses.




